Fig. 5
NMDA receptor activation and de novo protein synthesis are involved in the rapid downregulation of maturation markers by ECS. a, The relative expression of Calb1 in 5-HT4R-KO (-/-) mice at 6 h after a single ECS (*** P < 0.001, Bonferroni’s post hoc test following two-way ANOVA). b, The relative expression of Calb1 in mice treated with 5,7-DHT (i.c.v.) at 6 h after a single ECS (*** P < 0.001, Bonferroni’s post hoc test following two-way ANOVA). Veh: vehicle. c, The relative expression of Calb1 and Tdo2 at 6 h after a single ECS in mice treated with D-AP5 (1 μg/mouse, interaction [D-AP5 × ECS]; F (1,19) = 7.07 for Calb1, # P < 0.05, F (1,19) = 15.7 for Tdo2, ### P < 0.001). d, The relative expression of Calb1 and Tdo2 at 6 h after a single ECS in mice treated with cycloheximide (Chx; 200 mg/kg, i.p., interaction [cycloheximide × ECS]; F (1,11) = 11.35 for Calb1, ### P < 0.001, F (1,11) = 149.1 for Tdo2, ## P < 0.01). Bonferroni’s post hoc test following two-way ANOVA, *** P < 0.001, n.s., not significant. Sal: saline. The number (n) is shown in the graph. Data are presented as means ± SEM