Fig. 1

Yearlong study of motor function in Polr3a KI/KI and KI/KO mice. Results from the 12 mm (a, d) and 6 mm (b, e) beam test at four time points consisting of three trials per mouse. Latencies to cross (a, b) and number of foot slips (d, e) were recorded for both beam sizes. c, f) Results from the rotarod (c) and inverted grid (f) tests performed at three time points. The rotarod and inverted grid consisted of three trials per mouse. g-i) Results from the open field test performed at three time points. The open field test was run for 90 min per mouse during which total distance traveled (g), number of movements bouts (h) and total time spent moving (i) were recorded for each 10 min interval. The results represent the sum of all 10 min intervals. j-k) Results from gait analysis performed at the two latest time points. Paws were covered in color paint and mice were allowed to walk on a white paper-covered narrow runway. Distance between fore limbs and hind limbs was measured. All tests were performed on ≥14 female mice per group. For the beam test, rotarod and inverted grid, data are represented as adjusted least squares means +/− SEM of the sum of the three trials for each group. Groups were compared with one-way ANCOVA for each time point. #: p < 0.01