Fig. 4

Reducing neuronal activity using hM4Di/CNO significantly enhanced ketamine-induced neuronal apoptosis. a Schematic of the experimental procedure. b Representative confocal images of S1 sections from P9 mice labelled for CC3 (red), hM4Di-mCitrine (green), and Nissl (blue), conditions as indicated; scale bar is 200 μm. Zoomed images of boxed regions are presented to the right of each panel; scale bar is 50 μm. c Quantitation of the number of CC3⁺ cell per mm² S1, treatment conditions as indicated. hM4Di/CNO treatment significantly increased the number of CC3⁺ cells in the Keta + Dex group, but not in control PBS group. ^*** P < 0.001, n.s., not significant, using two-way ANOVA followed by Bonferroni post hoc test. d Proportion of hM4Di-mCitrine⁺ cells that are CC3⁺, conditions as indicated. hM4Di/CNO treatment significantly increased the percentage of CC3⁺ cells in Keta + Dex group, but not in the control PBS group. ^*** P < 0.001, n.s., not significant, using two-way ANOVA followed by Bonferroni post hoc test. e Distribution of CC3⁺ cells in different cortical layers. ^*** P < 0.001, n.s., not significant, using two-way ANOVA followed by Bonferroni post hoc test. 3 mice were used per condition, 10 brain slices from each mouse were quantitated. Data are shown as the mean ± SEM