Fig. 3

Effect of 5-HT on sEPSC recorded in neurons of the ACC. a Representative trace of sEPSC recorded in pyramidal neurons of the ACC layer II/III at a holding potential of −60 mV. b Cumulative probability plot showing the distribution of sEPSC amplitude in the phase of baseline, 5-HT application (5 μM) and washout.c Cumulative inter-events interval plot of recorded sEPSC in the phase of baseline, 5-HT application (5 μM) and washout. Black fill line indicated the phase of baseline, green fill line indicted the phase of 5-HT application and deep red dashed line indicated the phase of washout. d Summary result of averaged sEPSC amplitude (n = 15 neurons/7 mice). (E) Summary result of averaged sEPSC frequency (n = 12 neurons/7 mice). 5-HT (5 μM) application significantly reduced the frequency of sEPSC and washout partially reversed the reduction. ^** p < 0.01 compared to baseline; ^# p < 0.05 compared to the phase of 5-HT application