Fig. 4

Effect of 5-HT on mEPSC recorded in neurons of the ACC. a Representative mEPSC recorded in pyramidal neurons of the ACC layer II/III at a holding potential of −60 mV. b Cumulative plot of mEPSC amplitude of the phase of baseline, 5-HT application (5 μM) and washout. c Cumulative inter-event interval plot of recorded mEPSC in the phase of baseline, 5-HT application (5 μM) and washout. Black fill line indicated the phase of baseline, green fill line indicted the phase of 5-HT application and deep red dashed line indicated the phase of washout. d Summary plots of mEPSC amplitude (n = 12 neurons/6 mice). e Summary plots of mEPSC frequency (n = 10 neurons/6 mice). 5-HT (5 μM) application significantly reduced the frequency of mEPSC and washout partially reversed the reduction. ^** p < 0.01 compared to baseline; ^# p < 0.05 compared to the phase of 5-HT application