Fig. 2

Identification and localization of MGF. a In the skeletal muscle, MGF-positive cells are distributed along the basal membrane of the muscle fiber. b MGF is also co-localized in MyoD-positive satellite cells along the basal membrane. Red is MGF, Green is MyoD). c MGF is expressed in the peri-nuclear region of heart muscle cells. d at the gray/white junction of the cerebral hemispheres (dotted line), MGF+ neurons were found within the cortical gray matter, rather than in white matter (WM). e in the cortex MGF-positive neurons were round with very short processes. f In the lateral ventricular (LV) region, MGF was distributed along the wall of LV. g under higher magnification, MGF-positive cells exhibited long branched cellular processes like astrocytes. h In the hippocampus MGF positive cells were distributed at the border of the of the subgranular zone (SGZ)and dentate gyrus (dotted line) which is an area of active adult neurogenesis. i A small number of rounded MGF positive neurons were also observed in the striatum. j In the hypothalamus cells were detected in the paraventricular (V3) area. k Numerous cells were also seen in ventral pallidum area of the basal ganglia. (Red is MGF, Blue is DAPI, Scale bar, (in A-K) 100 um)