Fig. 3
EtOH-induced plasticity of GABAAR subunits and currents in rat after single-dose EtOH, CIE, and two-pulse EtOH. a: Summary of changes in mIPSCs, and b: inhibitory tonic currents after single-dose EtOH vs. pre-EtOH application (redrawn from Liang et al. [65]). A single dose EtOH induces loss of EtOH-sensitive tonic current and gain of EtOH-sensitive mIPSCs. Mean ± SEM are shown as % of vehicle-treated controls (red dashed line, n = 4–6. * p < 0.05). c: Biochemical analysis of GABAAR subunit plasticity in rat DG within 24 h after single-dose EtOH compared with the changes induced by CIE, 40-d withdrawal. Surface protein levels of GABAAR subunits measured using protein cross-linking and Western blotting. Mean ± SEM as % of vehicle-treated controls (red dashed line, n = 4–6. * p < 0.05). The α2 and γ1 subunits cell surface expression are up-regulated by both one-dose EtOH and CIE, γ1 total peptide is up-regulated, but not α2; and the heteropentameric subunit partnerships up-regulated are α4βγ2 and α2β1γ1. d, Upper panel: The protocol of double-dose EtOH experiment. d, Lower panel: Averaged mIPSC from each time point response to EtOH applications during the recordings. e: Summary of acute EtOH-induced changes in tonic current and mIPSCs (n = 5). f: Quantification of surface levels of GABAAR (n = 4–6) by Western blots for GABAAR α4 and γ1 after cross-linking in slices. g: Anxiety assayed by EPM (n = 6). The duration time rats stayed in arms (% of total 5 min). e,f,g: all bars are compared to the control (E0 value for that parameter): * p , 0.05; †p < 0.05. In e, the control level (dashed red line, at 100%) applies only to mIPSCs; in f, the red line refers to control (100%) for both subunits; in g, the dashed red line corresponds to the E0 point for either open or closed arms. c,d,e,f,g: from Lindemeyer et al., [30] with permission