Fig. 7

MiR-b2122 alters protein levels of TDP-43, FUS/TLS and RGNEF within a human neuronal cell line. Changes in protein levels of TDP-43, FUS/TLS and RGNEF were studied when SH-SY5Y cells were transfected with either miR-b2122, miR-b2122 plus its anti-miR, or the anti-miR alone. Transfection of let-7a was used as a negative control. a Western blot showing expression of TDP-43, FUS/TLS, RGNEF, and GAPDH (b) Quantification of Western blots for TDP-43, FUS/TLS and RGNEF protein levels. TDP-43 and FUS/TLS show small reductions in protein levels when transfected with miR-b2122 alone, while their protein levels increased when the anti-miR is added. Differences in protein levels when miR-b2122 or the anti-miR are added alone are significantly different for both TDP-43 and FUS/TLS. RGNEF has increased and decreased protein levels when either miR-b2122 or its anti-miR are added alone, respectively, and these differences are significantly different from one another. Protein levels were normalized to GAPDH. Data was expressed as the mean ± SEM, and significance was determined using a one-way ANOVA followed by a Tukey’s post-hoc (** = p < 0.01, * = p < 0.05)