Fig. 1
From: Direct interaction with 14–3-3γ promotes surface expression of Best1 channel in astrocyte
14–3-3γ interacts with Best1. a Identification of 14–3-3γ as a direct binding partner of Best1 in the yeast two-hybrid system. b Co-IP of GFP-Best1 with HA-tagged 14–3-3 isoforms in COS7 cells. Whole lysate of COS7 cells was immunoprecipitated with anti-HA antibody and then analyzed by Western blot with anti-GFP antibody. 5% of the total lysate was used as input for the immunoprecipitation. c Co-IP of Flag-14-3-3γ with GFP-Best1. Whole lysate of COS7 cells was immunoprecipitated with anti-Flag antibody and then analyzed by Western blot with anti-GFP antibody. 5% of the total lysate was used as input for the immunoprecipitation. d Representative confocal image of co-localization of Best1 and 14–3-3γ. GFP-tagged Best1 and mCherry-14-3-3γ were co-transfected in COS7 cell. Scale bar, 10 μm