Fig. 5
From: Direct interaction with 14–3-3γ promotes surface expression of Best1 channel in astrocyte
14–3-3γ-shRNA reduced Best1 mediated glutamate release from hippocampal astrocyte. a Representative measurement of glutamate current in a somatic region of CA1 pyramidal neuron of CaMKIIα-Cre mouse injected with adenovirus expressing control-shRNA or 14–3-3γ-shRNA under voltage clamp held at −60 mV during treatment with TFLLR (30 μM, grey bar). b Upper figures indicate fluorescent protein mCherry images of hippocampal CA1 region injected with adenoviruses transducing either control-shRNA or 14–3-3γ-shRNA. Bar graphs represent the averaged amplitudes (differences between baseline and TFLLR induced current amplitude depicted as dashed lines in (a)). Numbers of tested slices from at least two independent mice are indicated in the bar graph. Asterisk indicates a significant difference determined by one-way ANOVA test (*p < 0.05). c Traces from Ca2+ imaging recordings performed in naive astrocytes, pSicoR-control-shRNA expressing astrocytes, or pSicoR-14-3-3γ-shRNA expressing astrocytes. Each trace represents a Ca2+ response in one cell during treatment with TFLLR (30 μM, grey bar). d Summary bar graph for calcium imaging. Numbers of recorded cells are indicated on the bar graph