Fig. 3
From: Foxg1 deletion impairs the development of the epithalamus
Disrupted habenular innervations and abnormal diencephalic Chp and pineal gland. (a-b’): Immunofluorescence of L1 showing a reduced SM and habenular commissure (arrows in b and b’). Arrowhead indicates poorly fasciculated projections in the mutants compared with those in the controls. (c): Quantitative analysis of thickness of the habenular commissure at the midline area (n = 4, **p = 0.0015). (d, d’): less fasciculated FR and slightly changed projection angle. (e, e’): In situ hybridization of Ttr showing a more branched choroid plexus in the mutants. (f-i’): Immunofluorescence with Pax6 (f, f’) and in situ hybridisation of Otx2 (g, g’) and Fstl1 (h-i’) revealing an aberrant pineal gland, a shortened pineal stalk, which links the pineal gland to the habenula (i, i’, red broken line), and a lengthened Hbc area (h, h’, bracket; i, i’, yellow broken line). SM, stria medullaris; 3rdChp, third ventricle choroid plexus; LVChp, lateral ventricle choroid plexus; FR, fasciculus retroflexus; Hb, habenula; Hbc, habenular commissure; IPN, interpeduncular nucleus; Pg, pineal gland; Ps, pineal stalk. Scale bars: 100 μm (scale bar in g and g’: 400 μm)