Fig. 6

CCI induces a change in Beclin1 modulation in WT and TLR4 mice. a Beclin1 immunoreactivity was observed in the spinal dorsal horn of CCI mice. Compared with the contralateral side, a significant increase was shown in the ipsilateral side of WT mice, and no significant difference was found in TLR4 KO mice. Scale bar = 50 μm in A1, A3, A5, and A7. Scale bar = 20 μm in A2, A4, A6, and A8. b The number of Beclin 1 IR cells was significantly increased in the ipsilateral side compared with the contralateral side of WT mice. c The protein levels of Beclin1 were detected with immunoblotting. Western blot analysis indicated an increase in the levels of Beclin1 in the ipsilateral side compared with the contralateral side of WT mice. The band densities were analyzed with Image J and expressed as a percentage of the control. Two-way ANOVA; all the data are shown as mean ± SEM, where *P < 0.05 denotes a significant difference compared with the control group. d Frozen sections (WT-CCI, POD7) were stained with Beclin1 and co-stained with anti-GFAP (A1–4) anti-NeuN antibodies (B1–4). A4 and B4 is rectangular magnification of merged A3 and B3, respectively. Scale bar = 50 μm in A, B1–3. Scale bar = 20 μm in A, B4