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Fig. 7 | Molecular Brain

Fig. 7

From: TLR4-mediated autophagic impairment contributes to neuropathic pain in chronic constriction injury mice

Fig. 7

Activation of p62 in WT and TLR4 mice. a p62 immunoreactivity was observed in the spinal dorsal horn of CCI mice. Compared with the contralateral side, a significant increase in p62 IR cells was shown in the ipsilateral side of WT mice, and no significant difference was found in TLR4 KO mice. Scale bar = 50 μm in A1, A3, A5, and A7. Scale bar = 20 μm in A2, A4, A6, and A8. b The number of p62 IR cells was significantly increased in the ipsilateral side compared with the contralateral of WT mice. c Western blotting was performed to measure the protein expression of p62. Western blot analysis indicated an increase in the levels of p62 in the ipsilateral compared with the contralateral side of WT mice. The corresponding densitometric analysis is shown as bar graphs using Image J. Two-way ANOVA; all the data are shown as mean ± SEM, where *P < 0.05 denotes a significant difference compared with the control group. d Frozen sections (WT-CCI, POD7) were stained with p62 and co-stained with anti-GFAP (A1–4) and anti-NeuN antibodies (B1–4). A4 and B4 is rectangular magnification of merged A3 and B3, respectively. Scale bar = 50 μm in A, B1–3. Scale bar = 20 μm in A, B4

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