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Fig. 5 | Molecular Brain

Fig. 5

From: VGLUT1 or VGLUT2 mRNA-positive neurons in spinal trigeminal nucleus provide collateral projections to both the thalamus and the parabrachial nucleus in rats

Fig. 5

After the BDA injection into the Vo (a), Vi (b) or Vc (c), many BDA-labeled terminals in the ipsilateral PBN showed VGLUT2-LI (Vo: a1-a2; Vi: b1-b2; Vc: c1-c2). The site of BDA injection and the BDA anterogradely labeled terminals are visualized with fluorescein (green). VGLUT2-LI axon terminals are visualized with Alexa 594 (red), and neuronal cell bodies showing NeuN-LI are visualized with Alexa 647 (blue). The areas indicated by the short arrow in (a1), (b1) and (c1) are magnified in (a2), (b2) and (c2), respectively. The double arrowheads in (a2), (b2) and (c2) indicate axon terminals dually labeled with BDA and VGLUT2-LI (yellow). Scale bar 300 μm (a, b and c); 200 μm (a1, b1 and c1); 5 μm (a2, b2 and c2)

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Molecular Brain

ISSN: 1756-6606