Fig. 8
From: DNMT1 regulates expression of MHC class I in post-mitotic neurons
Knockdown of DNMT1 does not increase total MHC-I protein level on neurons detected by flow cytometry. CGN cultures were harvested and processed for flow cytometry, and analyzed the same day. Gating strategy is shown in A-C; a population experimentally determined to contain neurons were gated based on size (a), and from this population we proceeded with the live cells (b), from which the β-III-tubulin positive cells (neurons) were gated for further studies (c). D-F show MHC-I histograms for experimental groups gated on the neuronal population. Whereas treatment with IFNγ (blue) caused MHC-I expression on neurons, no MHC-I positive neurons were detected in untreated CGNs (purple), CGNs treated with non-targeting siRNA (green), or in CGNs treated with siRNA against DNMT1 (red) (d). Histograms of MHC-I signal in Fig. 8E-F show overlay of CGNs treated with non-targeting siRNA and IFNγ (light green), and CGNs treated with siRNA against DNMT1 and IFNγ (pink), and Fig. 8E shows the results from the cells receiving siRNA and IFNγ simultaneously, whereas Fig. 8F shows the results from the cells receiving IFNγ after the siRNA