Fig. 4
From: Dysregulation of human NEFM and NEFH mRNA stability by ALS-linked miRNAs
A group of ALS-linked miRNAs regulate a luciferase reporter linked to NEFM or NEFH 3’UTRs. HEK293T cells were co-transfected with a reporter plasmid containing NEFM or NEFH 3’UTRs and miRNA mimics. a, b Reporter gene assays were performed 24 h after transfection. Data are expressed as relative change and plotted in logarithmic scale. c, d Relative quantitative RT-PCRs were performed after RNA extraction, 24 h post-transfection. Data are expressed as relative mRNA level change and plotted in logarithmic scale. All experiments were performed in triplicate. Results are shown as mean ± SEM (Student t-test: **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, relative to the pmirGLO vector control). Reporter gene assays: miR-92a-3p/NEFM, p < 0.0001; miR-125b-5p/NEFM, p = 0.0005; miR-let-7a/NEFM, p = 0.6015; miR-9-5p/NEFH, miR-20b-5p/NEFH, miR-92a-3p/NEFH and miR-223-3p/NEFH, p < 0.0001; miR-519d-3p/NEFH, p = 0.0723; miR-let-7a/NEFH, p = 0.0893. RT-PCRs: miR-92a-3p/NEFM, p = 0.0052; miR-125b-5p/NEFM, p = 0.0429; miR-9-5p/NEFH, p = 0.0431; miR-20b-5p/NEFH, p = 0.0518; miR-92a-3p/NEFH, p = 0.0003; miR-223-3p/NEFH, p < 0.001; miR-519d-3p/NEFH, p = 0.0903)