Fig. 5

While neuronal chromatin appear non clustered under control conditions (a, c), NMDA-induced clustering of dark chromatin was prominent in neuronal nucleus from dissociated hippocampal cultures post-fixed with regular osmium tetroxide (b) but far less obvious in samples post-fixed with “reduced osmium” (1% ferrocyanide mixed with 1% osmium tetroxide, (d). Samples were sister cultures with the same NMDA treatment and processed in parallel. Scale bar = 0.5 μm