Fig. 3
From: A zinc-finger fusion protein refines Gal4-defined neural circuits
A combination of Crz-Zal1 and Crz-Gal4 drives expression in corazonergic cells. Maximum intensity projection (MIP) images of brain immunofluorescence. a–d MIP images of (A) of a UASG-mCD8GFP/+;Crz-Gal4/+ brain stained with α-GFP (green) and α-DLG (magenta) antibodies. b An image of a Crz-Gal4/UASZ-mCD8GFP brain stained with α-GFP (green), (C) and α-Crz antibodies (magenta) and (D) combined image. e–h UASZ-mCD8GFP/+; Crz-Zal1/+ brains stained with α-GFP, (e) α-DLG (magenta) and (h) α-Crz. i UASGZ-mCD8GFP/+;Crz-Gal4/+; Crz-ZAL1 brains stained with α-GFP and α-DLG (magenta) antibodies. j A Crz-Gal4/UASGZ-mCD8GFP brain stained with α-GFP, k α-Crz, and l combined image. m. Control brains were stained with α-GFP and α-Crz. Crz-Gal4 is inactive at non-cognate UASZ sites in Crz-Gal4; UASZ-GFP brains. n Crz-Zal1; UASG-GFP brains stained with α-GFP showed no green fluorescence. o Crz-Gal4; UASGZ-GFP brains showed no fluorescence. p Crz-Zal1; UASGZ-GFP showed weak expression in a few Crz cells (arrows indicate expression). Scale bar represents 200 μm; dorsal is up