Fig. 7
From: A zinc-finger fusion protein refines Gal4-defined neural circuits
In combination with different Gal4 lines, Trh-Zal1 defines distinct intersectional high-fidelity serotonergic neuronal sets. a-d. Expression patterns of UASG-GFP signal as driven from Gal4 lines: R22H10, R53C03, R70A11, and R89A09. Brain images show staining with α-GFP (green) and α-5HT (magenta) antibodies. a’-d’ The respective intersectional expression patterns when the drivers are used in combination with Trh-Zal1. a’ Intersectional expression from R22H10-Gal4 + Trh-Zal1 shows highly specific expression in serotonergic LP2 and SE3 cells (arrowhead) b’. Intersectional expression of R53C03-Gal4 + Trh-Zal1 shows specific expression in a few LP2, IP and SE1 serotonergic cells. c’. Intersectional expression of R70A11-Gal4 + Trh-Zal1 shows very specific serotonergic expression: two LP2, two PLP and IP cells. A few ectopic cells can also be seen in the subesophageal zone (SEZ). d’ Intersection of R89A09-Gal4 with Trh-Zal1 resulted in very specific expression pattern in the serotonergic SE3 cells