Fig. 4

CBD administration diminishes ischemic brain damage in wild-type but not σ1R^−/− mice. Upper panel, representative TTC-stained brain section images obtained from saline- and drug-treated mice (1 h after surgery) 48 h after pMCAO. White indicates infarction; red staining indicates normal tissue. Lower panel, the bar graphs quantitatively compare the infarct volume based on TTC staining from the wild type and σ1R^−/− mice treated with saline, the σ1R agonists PRE084 and PPCC (white bars), or the σ1R antagonist BD1063 and CBD (gray bars) 1 h after surgery. The groups consisted of 8 to 10 mice, and the data are presented as the mean ± SEM. *Significantly different from the saline-treated mice. φ Significantly different from mice receiving only the σ1R antagonist BD1063 or CBD; ANOVA, Dunnett’s multiple comparison vs the corresponding control group, p < 0.05