Fig. 1

The P-element insertion d03517 decreased male-male aggressiveness. a and b Intermale aggressive behaviors for 15-min period in wild-type and d03517 mutant flies. Prior to behavioral assays, d03517 flies were backcrossed with CS wild-type flies for four generations and thus were in w⁺ background. a Number of lunges. b Number of wing threats. Pairs of flies tested: wt, n = 27; d03517/ d03517, n = 27. Mann-Whitney U test, ^***P < 0.001. c Aggressive behaviours for 15-min period were examined when a wild-type male fly was paired with a d03517 mutant male fly. Pairs of flies tested: n = 46. Mann-Whitney U test, ^**P < 0.01. d Dominance index for successful occupancy of food patch by a wild-type or a d03517 mutant male fly after 10-min period. Pairs of flies tested: n = 46. Student’s t-test, **P < 0.01. Error bars represent SEM