Fig. 4
From: The peacefulness gene promotes aggression in Drosophila
pfs is broadly expressed in the adult male fly brain. a and b in situ hybridization detecting pfs mRNAs in an adult male fly brain. a Wild type. b pfsd03517 homozygous mutant. c Relative expression levels of pfs were quantified. Compared to that in wild type, the level of pfs mRNAs was significantly reduced in pfsd03517 homozygous mutants. Number of flies tested: wt, n = 7; pfsd03517, n = 7. Student’s t-test, *P < 0.05. Error bars represent SEM. d and e Confocal sectioning of fluorescence in male adult brains of w1118 control flies (d) and w1118 flies carrying GFP-tagged genomic pfs transgene (i.e. genomic-pfs-GFP) (e). d Autofluorescence background in w1118 control flies. e Pfs-GFP was broadly expressed in the adult brain of w1118 flies carrying genomic-pfs-GFP. Abbreviations: SMP, superior medial protocerebrum; AL, antennal lobe; SOG, suboesophageal ganglion. Scale bar: 50 μm