Fig. 6

pfs overexpression greatly increased intermale aggressiveness. Behaviours of a pair of male flies for 15-min period were examined. a Number of lunges. b Number of wing threats. c Number of tussles. Introduction of one copy of the pfs genomic rescue construct into wild-type flies did not increase the number of lunges or wing threats, but led to a small but significant increase in tussling, an intense fighting behaviour rarely observed in wild type. When two copies of the pfs genomic rescue construct were introduced into wild-type flies, all agonistic behaviours were significantly increased. Pairs of flies tested: wt, n = 22; 1× genomic construct, n = 27; 2× genomic construct, n = 25. Kruskal-Wallis and post hoc Mann-Whitney U tests, ^*P < 0.05, ^***P < 0.001. “ns”, not significant, P > 0.05. Error bars represent SEM