Fig. 2

Body maturation, motor function, and effects of rapamycin treatment on social interaction and body weight. (a) Valproic acid-exposed mice exhibited lower body weight on P18 and P25. (b) Valproic acid-exposed mice had lower eye-opening scores on P14. (c) Valproic acid-exposed mice exhibited a longer latency to right on P9 and P11 in righting reflex test. (d) Valproic acid-exposed mice exhibited a shorter latency to fall from P21 to P25 in the hanging wire test. (e) Social interaction test (5–6 weeks of age). Vehicle-treated VPA-exposed mice exhibited a decrease in active social interaction time compared with vehicle-treated control mice. Rapamycin-treated VPA-exposed mice exhibited an increase in active social interaction time compared with vehicle-treated VPA-exposed mice. (f) Social interaction test (10–11 weeks of age). Vehicle-treated VPA-exposed mice exhibited a decrease in active social interaction time compared with vehicle-treated control mice. Rapamycin-treated VPA-exposed mice exhibited an increase in active social interaction time compared with vehicle-treated VPA-exposed mice. (g) No significant difference in body weight was found between vehicle treatment and rapamycin treatment. Administration of rapamycin for 2 consecutive days did not affect body weight. Significant differences in body weight were found between control and VPA-exposed mice in both adolescence and adulthood. Error bars indicate SEM. *p < 0.05, **p < 0.01, ***p < 0.001