Fig. 2
From: Hyperexcitability of the local cortical circuit in mouse models of tuberous sclerosis complex
Surface biotinylation assay of dissociated Syn1-Cre x Tsc1fl/fl mouse cortical neurons. a Surface receptors were biotinylated in cultured Syn1-Cre x Tsc1fl/fl mouse cortical neurons and pulled down using neutravidin-agarose slurry. Representative immunoblots of GluA2, GABAAR-α and transferrin receptor are shown. b, c Band intensities of GluA2 and GABAAR-α are normalized to the transferrin receptor. While the mutant neurons showed GluA2 band intensity ratio comparable to control, they had a significant reduction in GABAAR-α band intensity ratio. * P < 0.05 in (c). Error bars represent s.e.m. in (b) and (c)