Fig. 4
From: Quantitative profiling brain proteomes revealed mitochondrial dysfunction in Alzheimer’s disease
Regulation of NDUFA4, NDUFAB1 and NDUFA9 in early onset AD. a iTRAQ quantitative abundances of NDUFA4, NDUFAB1 and NDUFA9 in early onset AD, b Western blot analysis of NDUFA9 and NDUFAB1 using mouse monoclonal anti-NDUFA9 and rabbit monoclonal anti-NDUFAB1 that were purchased from Abcam, c and d Quantitative analysis of immunoblot images by software ImageJ. Differences between means were assessed with the one-way ANOVA test. P-value < 0.05 was considered to indicate statistically significant differences. e Label free quantitative abundances of subunits NDUFA4, f Label free quantitative abundances of subunits NDUFAB1, g Label free quantitative abundances of subunits NDUFA9. The dot indicates values in individual brain sample, their biological and technical replicates). Brain mitochondria was isolated, purified using anti-TOM22 magnetic beads and analyzed using Q-Exactive mass spectrometry