Fig. 5
From: Quantitative profiling brain proteomes revealed mitochondrial dysfunction in Alzheimer’s disease
Regulation of NDUFA4, NDUFAB1 and NDUFA9 in late onset AD. a iTRAQ quantitative abundances of NDUFA4, NDUFAB1 and NDUFA9 in late onset AD, b Western blot analysis of NDUFA9 and NDUFAB1 using mouse monoclonal anti-NDUFA9 and rabbit monoclonal anti-NDUFAB1 in individual samples of control and late onset AD, c and d Quantitative analysis of immunoblot images by software ImageJ. e Label free quantitative abundances of subunits NDUFA4, f Label free quantitative abundances of subunits NDUFAB1, g Label free quantitative abundances of subunits NDUFA9. The dot indicates values in individual brain sample, their biological and technical replicates. Brain mitochondria was isolated, purified using anti-TOM22 magnetic beads and analyzed using Q-Exactive mass spectrometry