Fig. 7

Sacsin expression in CNS neurons. (a-k) Immunohistochemistry using antibody against sacsin on sagittal brain sections from 300 day-old control mice demonstrate the widespread sacsin expression in several CNS populations. Sacs^−/− mice were used as negative controls (a’-k′). The highest expression of sacsin is seen in the cerebellum, pons and medulla. Cerebellar PC cell bodies, dendrites (a) and axons (asterisk in b) are highly labeled, a subpopulation of DCN neurons are highly labeled (pink arrows in b), whereas other DCN neurons are lightly labeled (yellow arrowheads in b). The same is observed in the pons, some isolated neurons are highly labeled (pink arrows in c), whereas others are lightly labeled (yellow arrowheads in c). In the thalamus, sacsin labeling seems restricted to the neuritis with little labeling in the neuronal cell bodies (d). Fiber tracts in the corpus callosum are positive for sacsin immunolabeling (e). Neurons in the superior olivary complex (SOC) show sacsin immunolabeling (f). Sacsin immunolabeling is observed in the cell bodies and dendrites of neurons in the isocortex visual (arrows in g) and motor areas (arrows i). In the somatosensory area of the isocortex, sacsin immunolabeling seems more in the neurites than in the neuronal cell bodies (h). Olfactory bulb mitral cells are also sacsin positive (j), and light labeling is observed in the hippocampal formation pyramidal cell neurites (k). Scale bar in K′ = 100 μm