Fig. 3

Differentially expressed proteins between WT and NPC mice. a: Volcano plot of the Log2 LFQ differences versus -Log10 P-values of the common proteins between WT and NPC. The downregulated proteins in NPC were on the left side and significant ones were labeled in red; the upregulated proteins were on the right and significant ones were labeled in red. b: The Go CC, biological processes (Go BP) and Reactome pathway (Reactome) enrichment of differentially expressed proteins, the names of protein in each term were listed on the bars, the red point marked FDR value presented by its -Log10 values. c, d: Western blots (c) and quantification (d) of the differentially expressed proteins from corpus callosum of mice. The value of each protein was normalized to the corresponding loading control and the values of WT were set to 1. Three biological replicants were compared and GAPDH was used as the loading control. Student’s t-test was used. *** p < 0.001