Fig. 4
Prenatal FLX-induced deficits in executive function are rescued by a 5-HT2AR antagonist. (a) Schematic diagram of the drug treatment procedure. Prenatally SAL-treated mice were injected with vehicle (VEH), whereas prenatally fluoxetine (FLX)-treated mice were randomly assigned to VEH and 5-HT2AR antagonist (MDL) groups. Thirty minutes before behavioral testing, FLX mice received i.p. injections of either vehicle (0.5% DMSO) or MDL (0.01 mg/kg in 0.5% DMSO). (b) Bar plot of spontaneous alternation rates of SAL-treated mice that were administered VEH (black) and FLX-treated mice that were administered VEH (red) or MDL (blue) in the Y-maze. (c) Bar plot of the total number of entries into all arms of the Y-maze. (d) Representative heat map of results of the three-chamber social interaction test with a novel mouse and object. The occupancy rate was normalized to the region with peak occupancy in the arena. (e) Bar plot of the times spent sniffing the novel mouse and object in the three-chamber social interaction task. (f) Bar plot of the social preference index (time spent sniffing the mouse/total time spent sniffing the mouse and the object). The dotted line indicates an equal preference (50%) (g) Representative heat map of results from the three-chamber social interaction test with a familiar mouse and novel mouse. (H) Bar plot of the times spent sniffing the familiar mouse and a novel mouse in the three-chamber social interaction task. (i) Bar plot of the social novelty preference index (time spent sniffing the novel mouse/total time spent sniffing both mice). Data are presented as mean ± SEM. (e) and (h) Two-way repeated measures ANOVA. (b), (c), (f) and (i) One-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001