Fig. 5

Directional migration of hG008 cells in vivo. Brain sections from mice implanted with hG008 cells were immunostained with anti-Neurofilament protein, anti-Nestin, anti-GFAP, anti-O4, anti-NeuN, and anti-mouse CD31 antibodies. a Migration along axonal fibers in the striatum, transitional zone between striatum and corpus callosum, corpus callosum, and cingulate gyrus is observed. Scale bar, 100 μm. b H&E staining and fluorescence images are shown. Directional migration toward the inferior horn of the lateral ventricle is observed. Scale bar, 100 μm. c Localization in the perivascular area was analyzed by immunostaining for CD31, an endothelial cell marker. Scale bar, 100 μm