Table 1 Primers used in real-time PCR analyses
From: Predictable chronic mild stress promotes recovery from LPS-induced depression
Target gene | Â | Primers sequences | Size (bp) |
|---|---|---|---|
β-Actin | Forward | 5′-GGGAAATCGTGCGTGAC-3′ | 176 |
Reverse | 5′-AGGCTGGAAAAGAGCCT-3′ | ||
IL-1β | Forward | 5′-ATGTGCTGCTGCGAGATTTGA-3′ | 136 |
Reverse | 5′-TGCCACCTTTTGACAGTGATG-3′ | ||
IL-6 | Forward | 5′-TTGGGAGTGGTATCCTCTGTGAA-3′ | 88 |
Reverse | 5′-CCTTCTTGGGACTGATGCTGG-3′ | ||
TNF-α | Forward | 5′-CACCCCGAAGTTCAGTAGACA-3′ | 184 |
Reverse | 5′-TCCCTCCAGAAAAGACACCAT-3’ | ||
Bax | Forward | 5′-AGGATGCGTCCACCAAGA-3’ | 194 |
Reverse | 5′-AAAGTAGAAGAGGGCAACCAC-3’ | ||
Bcl-2 | Forward | 5′-CGGGAGAACAGGGTATGA-3’ | 149 |
Reverse | 5′-CAGGCTGGAAGGAGAAGAT-3’ | ||
NLRP3 | Forward | 5′-ATGTTGCCTGTTCTTCCA-3’ | 130 |
Reverse | 5′-GGGTCTCCCAGAGTATTG-3’ | ||
ASC | Forward | 5′-AGCAAGAGTAAAAGGTGACCG-3’ | 103 |
Reverse | 5′-TTGAGTTCATCCCCTGACAAG-3’ | ||
Nrf2 | Forward | 5′-CAGTGCTCCTATGCGTGAA-3’ | 109 |
Reverse | 5′-GCGGCTTGAATGTTTGTC-3’ | ||
HO-1 | Forward | 5′-ACAGAGGAACACAAAGACCAG-3’ | 136 |
Reverse | 5′-GTGTCTGGGATGAGCTAGTG-3’ | ||
NQO1 | Forward | 5′-TACGATCCTCCCTCAACA-3’ | 108 |
Reverse | 5′-TACAGCAGCCTCCTTCAT-3’ |