Fig. 3
From: NMDAR mediated translation at the synapse is regulated by MOV10 and FMRP
Translation of specific mRNAs is affected on MOV10 knockdown. a Immunoblots showing MOV10 knockdown from primary neuronal cultures transfected with scrambled siRNA (scr-siRNA) or MOV10 siRNA (MOV10-siRNA). Quantification of MOV10 in from primary neurons transfected with scramble siRNA (scr-siRNA) or MOV10 siRNA (MOV10-siRNA). Values normalised to samples from scrambled siRNA transfected cells (n = 3, unpaired Student’s t-test, ±SEM). b Distribution of RPLP0 on a linear sucrose gradient from primary neurons transfected with scrambled or MOV10 siRNA based on immunoblots shown below. c-f Distribution of selected mRNAs on linear sucrose gradient from primary neurons transfected with scrambled siRNA (scr-siRNA) or MOV10 siRNA (MOV10-siRNA) followed by quantification of mRNAs in polysomes (bar graphs, n = 4–6, unpaired Student’s t-test, ±SEM) for the mRNAs Pten (c), Psd-95 (d), Ank2 (e) and b-actin (f). Also see Additional file 1: Figure S4D-S4G