Fig. 1

Fewer neuron and parvalbumin (PV)-interneuron numbers in the Fmr1 KO mouse cortex. a Representative fluorescent images of NeuN-immunostained cells outlining the regions of interest across the neocortex, which includes the cingulate, motor and somatosensory cortex in WT and Fmr1 KO mice. Scale Bar: 300 μm. b-d Higher magnification images for each region are correspondingly shown. Scale Bar: 100 μm. e, f The number of NeuN-positive cells in the cingulate cortex of KO mice is significantly lower than WT, but not the motor or somatosensory regions (n = 24 per group). g, h Immunolabeling of parvalbumin (PV) shows that Fmr1 KO mice also have less PV-interneurons in the neocortex when compared to WT (n = 20–24 per group). Scale Bar: 100 μm. Data are presented as mean ± SEM. **p < 0.01. Cg: cingulate cortex, MC: motor cortex, SsC: somatosensory cortex