Fig. 4
From: Altered cortical Cytoarchitecture in the Fmr1 knockout mouse
Fmr1 KO mice have less Iba1-positive microglia but more GFAP-astrocytes. a WT and Fmr1 KO mouse cortices are immunostained with Iba1 for microglia analysis. Scale Bar: 100 μm. b Quantification of Iba1-positive cells reveals fewer numbers in Fmr1 KO mutants (n = 46–48 per group). c Anti-GFAP is used to label astrocytes in the corpus callosum. Scale Bar: 100 μm. Higher magnification images are shown on the right. Scale Bar: 50 μm. d Fmr1 KO mutants have substantially more GFAP-astrocytes and higher GFAP expression (n = 14–16 per group). Data are presented as mean ± SEM. *p < 0.05