Fig. 4

Normal myelination and cerebellar integrity in double mutant mice at 6 months of age. a Immunofluorescence for proteolipid protein (PLP) showing normal staining in the corpus callosum (top: coronal view; bottom: sagittal view) of DM mice compared to WT. Staining was performed on three mice per group and representative images are shown for each group. Scale bar: 100 μm. b Top: Nissl staining of sagittal cerebellar sections performed on two mice per group. Representative images are shown for each group. Scale bar: 100 μm. Bottom: Purkinje cell counts of mid-sagittal cerebellar sections. Data are represented as mean +/− SEM of three sections per mouse