Fig. 5

Normal expression of Polr3a and Polr3b mRNAs and Pol III transcripts in double mutant mice at 6 months of age. a, b Expression levels of a) Polr3a and Polr3b and b) three Pol III transcripts measured by RNA-seq in the cerebrum of three WT and three DM mice. Expression was normalized with DESeq2. Data are represented as mean +/− SEM. c Northern blots of precursor (pre) and mature tRNA species from the cerebrum (left) and cerebellum (right) of three WT and three DM mice. U3 snRNA, a Pol II transcript, was used as a loading control. Quantification of mean +/− SEM after normalization are indicated below the blots for each group