Fig. 5

Reduced cell proliferation in the dorsal telencephalon of LIMK KO mice. a Pregnant mice were injected with BrdU at E14.5 and 2 h later the embryos were dissected and stained for BrdU. b Magnified views of the boxed regions in (a). c Summary graph of (a) showing a significant reduction of BrdU⁺ cells in the dorsal VZ in various LIMK KO embryos. d Coronal brain sections of E14.5 embryos stained for TUNEL (green) and DAPI (blue). e Summary graph showing significantly increased numbers of TUNEL positive cells in LIMK2 KO and LIMK1/2 DKO mice. Control, n = 4; LIMK1 KO, n = 5; LIMK2 KO, n = 3; LIMK1/2 DKO, n = 3. Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA, Dunnett’s test. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars, 50 μm