Fig. 3
From: SUMOylation regulates USP5-Cav3.2 calcium channel interactions
SUMO-resistant USP5-K113R shows enhanced Cav3.2 / USP5 interactions. a. Representative western blots of Cav3.2-GFP tagged immunoprecipitates showing increased binding to USP5-K113R mCherry tagged mutant. The blot was probed with an mCherry antibody. b.USP5-WT and the USP5-K113R mutant show equal levels of expression as detected by immunoprecipitation followed by western blot using an mCherry mouse antibody. c. Cav3.2-GFP tagged immunoprecipitates showed similar levels of expression when co-expressed with mCherry-tagged USP5-WT or mCherry tagged USP5-K113R mutant as detected by western blot using a GFP rabbit polyclonal antibody. d. Actin control using an actin antibody to probe whole cell lysates expressing either Cav3.2-GFP + USP5-WT (left lane) or Cav3.2-GFP + USP5-K113R (right lane). e. Densitometry analysis of USP5-WT or USP5-K113R bound to Cav3.2-GFP immunoprecipitates. Numbers in parentheses reflect numbers of separate experiments, asterisks denote statistical significance at the 0.01 level