Fig. 4

Nsun5 deficiency disrupts the radial glial scaffolds. a & b The development of radial glial scaffolds was compared between E12.5-E18.5 WT mice and Nsun5-KO mice. Images of nestin (green) or BLBP (red) immunostaining. Scale bars, 50 μm (a); 25 μm (b). Many long, parallelly distributed nestin+ or BLBP+ processes span throughout the cortical wall in WT mice. In E14.5-E18.5 Nsun5-KO mice, the radial processes are discontinuous and shorter (open arrows). c High magnification images of BLBP labeling at E14.5. Scale bars, 10 μm. In WT mice the end-feet of RGC scaffolds attached to the pial BM, but in Nsun5-KO mice many RGC scaffold end-feet detached from the pial BM (arrows). d & e Representative images of Sox2 at E14.5 or Tbr2 at E16.5. Scale bars, 25 μm. Bar graphs show the numbers of Sox2+ cells and Tbr2+ cells in WT mice and Nsun5-KO mice