Fig. 5
From: Expression of the RNA methyltransferase Nsun5 is essential for developing cerebral cortex
Nsun5 deficiency affects the radial migration of cortical neurons. a Representative images of BrdU immunostaining at E12.5, arrows indicate BrdU+ cells, Scale bars, 25 μm. Bar graphs show the numbers of BrdU+ cells. b & c The cortical distribution of E12.5-E18.5 BrdU+ cells and E14.5-E18.5 BrdU+ cells was compared between WT mice (WT) and Nsun5-KO mice (KO). Representative images of immunostaining for BrdU in E18.5 cerebral cortices. Scale bars, 50 μm. Bar graphs (in middle column) show the total number of E12.5-E18.5 BrdU+ cells per section (upper panel) and E14.5-E18.5 BrdU+ cells (bottom panel). *P < 0.05 vs. WT mice. The percentage (%) of E12.5-E18.5 BrdU+ cells (upper panel) or E14.5-E18.5 BrdU+ cells (bottom panel) located in the upper-layers, deeper-layers and subcortical region was showed in the bar graphs (in right column). *P < 0.05 and **P < 0.01 vs. WT mice. d Representative images of cleaved caspase-3 immunostaining. Scale bars, 50 μm. Bar graph shows numbers of caspase-3+ cells (arrows) in E18.5 WT mice and Nsun5-KO mice. *P < 0.05 vs. WT mice