Fig. 2

Localization of e37a-Cacna1b mRNA in vHPC, mPFC, neocortex and AMY. a Schematic depicting BaseScope™ workflow used to detect e37a-Cacna1b mRNA in brain sections. b Representative BaseScope™ images of vHPC from sections of WT mice. c mPFC section from WT and e37b-only mice, left and right respectively. d Bottom left and right show representative images from AMY of sections from WT mice. In b-d, middle panels indicate localization of nuclei stained with e37a (red dots). These red dots were constructed relative to the image shown in the corresponding left panel. Right panel shows amplified insets from left panel. e Comparisons of nuclei containing signal for e37a (e37a⁺) between WT and e37b-only mice. Percent e37a⁺ nuclei was calculated by normalizing the number e37a⁺ nuclei to all nuclei stained with hematoxylin in cortical sections of mice from both genotypes. Data are represented as mean (filled symbols) ± SE and individual values (empty symbols). * p < 0.05