Table 1 Primers and expected products

CCK-Cre

F-WT1: GGGAGGCAGATAGGATCACA

F-MT1: TGGTTTGTCCAAACTCATCAA

R: GAGGGGTCGTATGTGTGGTT

Hom: 180 bp

Het: 180 bp and 468 bp

WT: 468 bp

Dlx5/6-Flpe

F-T1: CAGAATTGATCCTGGGGAGCTACG

R-T1: CCAGGACCTTAGGTGGTGTTTTAC

F-C: CAAATGTTGCTTGTCTGGTG

R-C: GTCAGTCGAGTGCACAGT TT

Transgene: 406 bp

PCR positive control: 200 bp

*PCR conditions do not differentiate between heterozygous and homozygous mice

Ai14 and RCFL-tdT

F-WT2: AAGGGAGCTGCAGTGGAG TA

R-WT2: CCGAAAATCTGTGGGAAG TC

R-MT1: GGCATTAAAGCAGCGTAT CC

F-MT2: CTGTTCCTGTACGGCATGG

Hom: 196

Het: 196 and 297

WT: 297

CaMKIIα-Cre

F-T1: GTT CTC CGT TTG CAC TCA GG

R-T1: CAG GTT CTT GCG AAC CTC AT

F-C: AGT GGC CTC TTC CAG AAA TG

R-C: TGC GAC TGT GTC TGA TTT CC

Transgene: ~  500 bp

PCR positive control: 521 bp