Fig. 4
From: miR-212-5p attenuates ferroptotic neuronal death after traumatic brain injury by targeting Ptgs2
miR-212-5p directly targets Ptgs2 in HT22 and Neuro-2a cell lines. Schematic diagram of strategy to discover the target gene of miR-212-5p is shown in (a). b The expression of Ptgs2 was significantly increased at all indicated time points after CCI. c Sequence alignment of miR-212-5p and the 3′-UTR of Ptgs2. The seed sequence of miR-212-5p and the binding sites in 3′-UTR are marked with underscores. d miR-212-5p overexpression inhibited the expression of 3′-UTR-luciferase reporter of Ptgs2 in HT-22 and Neuro-2a cells, but the mutant construct was immune to miR-212-5p. The 3′-UTR mutants containing mismatched nucleotides are shown at the bottom. In HT-22 (e) or Neuro-2a (f) cells, miR-212-5p overexpression inhibited the expression of Ptgs2, while inhibition of miR-212-5p caused the downregulation of Ptgs2. Data are presented as mean ± SEM from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001