Fig. 6

MAP 1B phosphorylation at S25 and at S1201 are induced and maintained in the proximal segment of the transected sciatic nerve. a, b, c and d Immunofluorescent staining for pS25 (a), pS1201 (b), pan-MAP 1B (c), or SCG10 (d) with Tuj-1 Abs of intact nerve (left), transected nerve on 1 day (middle), and 3 days (right) after injury. On day 1 and day 3, the proximal and the distal segments were located in the left and the right side from the injury point. Scale bar; 200 μm. e, f, g and h Quantitative assessment of the signal intensity of pS25 (e), pS1201 (f), pan-MAP 1B (g), and SCG10 (h). N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001, by comparison with that in the intact nerve; using one-way ANOVA with Dunnett’s multiple comparisons test