Fig. 5
From: Pathophysiology of and therapeutic options for a GABRA1 variant linked to epileptic encephalopathy
Insulin or diazepam or their combination could only partially rescue the functional deficits of R214C GABAARs. a Representative traces of GABA (10 μM, 1 s)-evoked currents from WT or R214C GABAAR expressing cells, with or without rapid diazepam application (1 μM, 1 s). b Quantification of averaged peak current amplitudes recorded from cells expressing WT (n = 13) or R214C (n = 15) GABAARs before and after exposure to diazepam. c Representative traces of GABA-evoked currents from WT or R214C GABAAR expressing cells, with or without insulin (0.5 μM, 10 min) treatment. Cells were first serum starved for 2 h prior to recording, and GABA currents were then evoked before and after treatment of insulin for 10 min. d Quantification of averaged peak current amplitudes recorded from cells expressing WT (n = 8) or R214C (n = 6) GABAARs before and after insulin treatment. e Representative traces of GABA-evoked currents from WT or R214C GABAAR expressing cells before and after insulin and diazepam co-treatment. Cells were first serum starved for 2 h before recording an initial GABA-evoked current (10 μM GABA, 1 s). The same cell was then perfused with insulin (0.5 μM, 10 min) in the recording chamber, and a second GABA-evoked current (1 μM diazepam, 0.5 μM insulin, 10 μM GABA, 1 s) was recorded thereafter. f Quantification of averaged peak current amplitudes recorded from cells expressing WT (n = 12) or R214C (n = 11) GABAARs before and after insulin and diazepam co-treatment. Statistical differences were determined using paired t-test (*p < 0.05, **p < 0.01, ***p < 0.001). Data is represented as +SEM