Fig. 7
From: Pathophysiology of and therapeutic options for a GABRA1 variant linked to epileptic encephalopathy
Verapamil treatment increased total, but not surface expression levels of α1 subunit in R214C GABAAR expressing cells. a Representative whole cell lysate blots from WT and R214C GABAAR expressing cells. b Quantification of total expression levels of α1 subunit in untreated or verapamil treated (4 μM, 24 h) WT and R214C GABAAR expressing cells (n = 9). c Representative surface expression blots from WT and R214C GABAAR expressing cells. d Quantification of surface expression levels of α1 subunits, normalized to Na+/K+ ATPase expression levels, and represented as a fold change against untreated WT GABAAR expressing cells (n = 13). Statistical differences were determined using two way ANOVA followed by post hoc student’s t-test by comparing to expression levels of untreated WT expressing cells (*p < 0.05, ***p < 0.001), or untreated R214C expressing cells (#p < 0.05). Data is represented as +SEM