Fig. 5

Marker genes for neurons expressing both Th and Slc17a6 in the VTA. (a) Virtual expression profile for a marker candidate of neurons expressing both Th and Slc17a6. The virtual profile was defined as the minimum (red) Slc17a6 (green) and Th (blue) expression intensity. Gray shading indicates the minimum expression level between Th and Slc17a6. The blue triangle indicates a guide point for designation of medial and lateral (voxels 1–26 and 27–42, respectively). (b) Heat map showing that the expression of the selected candidates is similar to that of the virtual expression profile. Expression intensities for each gene were autoscaled to yield a mean of 0 and a standard deviation of 1 (red, positive; blue, negative). The blue triangle indicates a guide point for medial and lateral as for panel a. (c) List of selected top five marker candidate genes for Th-Slc17a6 co-localized cells in the VTA (final selected gene is in red). (d) ISH images showing expression of Slc17a6 and Wwox in the VTA