Fig. 5
From: Plasma membrane localization of MLC1 regulates cellular morphology and motility
Interaction between MLC1 and ARP2/3 complex. a and b FLAG-MLC1-GFP, ARP3-V5, and ARP3-Myc were co-expressed in COS-7 cells and used for co-immunoprecipitation. MLC1 and ARP2 or 3 were immunoprecipitated with anti-FLAG, V5, Myc antibodies, respectively. MLC1 was detected in V5- and Myc-precipitants, additionally, ARP3 and ARP2 were detected in FLAG-precipitant. Actin was used as loading control. c Primary astrocytes were infected with rAAV-GFP-shScr or -shMlc1. Endogenous Arp3 and Cortactin were immunoprecipitated with specific antibodies (Input = 10% of IP)