Fig. 6
Knockdown in the eye disc only or in WG only. For eye-specific knockdown, UAS-RNAi transgenes were under control of ey3.5-GAL4. For WG-specific knockdown, UAS-RNAi transgenes were under control of Mz97-GAL4. 3rd-instar larval eye-brain complexes were double-stained with MAb24B10 (green) and anti-Bdl (magenta). A-A”, both R cells and WG developed normally when ama was only knocked down in WG. B-B″, knocking down ama in the eye disc only caused a phenotype identical to that in animals when ama was knocked down in both eye disc and WG. C-C″, unlike that in animals in which nrg was knocked down in both eye disc and WG (Fig. 4E-E”), the development of R cells and WG appeared normal in animals in which nrg was only knocked down in WG. D-D”, the development of R cells and WG occurred normally in animals in which nrg was only knocked down in the eye disc. Scale bar: 20 μm